After we reported the use of fresh tumor tissues removed surgically for therapeutic purposes in 1994, the
removed tumor tissues have been largely applied for clinical treatment from a level of fresh tissues, level
of frozen tissues until the level of pathological slides. Because the cell components from fresh tumor
tissues are easier to culture than those frozen in liquid nitrogen tanks, both primary tumor cells and fresh
immune cells from fresh tumor tissues are going to be increasingly researched for clinical applications,
including precision medicine. This article will focus on the current progress of the landscape and
architecture of freshly resected tumor tissues for precision medicine. Relying on the increasing reports
of precision medicine, this article aims to report the landscape and architecture of tumor tissues for
precision medicine of tumor diseases. This manual introduces the landscape and architecture of fresh
tumor tissue gross specimens, the landscape and architecture of 3-D tumor tissue culture, the landscape
and architecture of TME in tumor tissues, and finally, the landscape and architecture of tumor tissues
can combine with single-cell technology, clinical genomics, and artificial intelligence so that the tumor
tissue landscape and structure with clinical genomics and single-cell technology will allow us to find
more specific and sensitive treatment for tumor diseases than our earlier established model of TIL
infusion and ex vivo chemotherapy sensitivity testing (CST) from the resected tumor tissue.
